Yeast washing

[brewboy4]

So I captured some yeast with left over wort from a previous brew day. I boiled shrimp me hops then set it out in the garden for a few days. It started fermenting after a few days so I decided to try isolate 1 strain. So I have got the yeast here but what part of the liquid in the jar is yeast



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[nbpicklesno2]

So I captured some yeast with left over wort from a previous brew day. I boiled shrimp me hops then set it out in the garden for a few days. It started fermenting after a few days so I decided to try isolate 1 strain. So I have got the yeast here but what part of the liquid in the jar is yeast

This was quite possibly the most confusing post I have ever read. It does raise some questions.

How did you capture yeast from wort?
What are 'shrimp me' hops?
Why did you put them in the garden for several days?
Was there more than one strain of yeast used in the first place and is that why you were trying to isolate one strain?

From the pictures it's hard to tell what you have. If this is from a previous brew, the watery bit on top is the yeast and the bottom solid bit is trub. If you put it in the fridge for 24 hours, the yeast will sink to a layer at the bottom of the jar.

It may just be me, and I may be extraordinarily stupid, but your turn of phrase seems a little.....eccentric.

[hazelbrew]

I would have said the turn of phrase seems very "autocorrected"

[McMullan]

If you want to isolate wild yeast, you'd be better off using agar plates and picking yeast colonies from them. Oak trees are the best place (natural habitat) to find strains suitable for brewing. You'll have all kinds of bugs in that wort

[Fil]

yeast washing referes to washing with an acid, yeast rinsing looks like what your after doing..

let the murk all settle out, the yeast will form a creamy off white layer without a granular look...

[nbpicklesno2]

I would have said the turn of phrase seems very "autocorrected"

I think it's a little beyond auto correction

[brewboy4]

I've always known it as yeast washing . Anyway I do plan on plating it upon some ydp media which is the 1st stage of my selection media. I just wanted to know what layer was the yeast


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[Jocky]

As I understand it, you've used some left over wort, boiled it up with hops and then left it out in the garden and caught some wild yeast in it.

At this point you have two choices:

1. Create another starter to try and step up whatever you've captured.

2. Streak it out on an agar plate to try and properly isolate your wild yeast.

I would go with #1, but it looks like you have trub left in your old wort that will make this tricky, as you won't have much yeast in there at all.
Stick your wort in the fridge for 48 hours to drop out all the yeast. Then pour off the liquid before pitching the rest into a 500ml starter. Repeat again with 1 litre and then again with 2 litre and you should be getting toward pitchable amounts of yeast as well as knowing from tasting the starter whether it's producing anything good.

[Fil]

I've always known it as yeast washing . Anyway I do plan on plating it upon some ydp media which is the 1st stage of my selection media. I just wanted to know what layer was the yeast


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ahh crossed wires,, if your liquor is teaming with yeast simply dip your sterile loop in and streak your plate with it 3 x overlapping zig-zags that should isolate individual cells to develop into colonies,

you shouldnt need to dig into a layer of yeast for this, if the liquor has lost significant gravity due to yeast activity its teeming with the lil buggers, Billions of em.... mix up the mixture and dip a loop in and it will pick up a few hundred easily..

though if its your first time, prep a few practice plates too.. i ruined half a dozen with heavy handedness..


If you think you have picked up bacteria in your liquor then washing with an acid solution will kill the bacteria which if streaked onto a plate will probably dominate,

My 'bible' on the subject..
https://www.google.co.uk/url?sa=t&rct=j ... 8493,d.d2s

[brewboy4]

I am planing on doing and acid wash . Lemmon juice and water solution 2.2 ph for 4 hours then I will streak the plates


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[McMullan]

I am planing on doing and acid wash . Lemmon juice and water solution 2.2 ph for 4 hours then I will streak the plates

Acid washing is an involved protocol used for reducing bacterial contamination in economically significant volumes of pitchable yeast slurry. Considering any application for home brewers is silly, TBH. Acid washing is completely pointless in this situation and is more likely to have a negative impact on already stressed yeast cells. Go straight to agar plates. You'll probably have to re-streak once or twice to culture something pure. Note that most wild Sacc spp are not suitable for brewing. Finding one that is is a genuine achievement. What's your motivation for doing this anyway?

[brewboy4]

I'm just doing for a bit of fun l. I plan on doing some selectivity agar to find something suitable. It hasent really cost me anything to do money wise and only a few minutes a day. I had a taste of the "beer" from the spontaneous fermentation and it tasterf ok so I hope get something intresting


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[brewboy4]

So I made my agar plates with some agar,dme and yeast nutrient then streaked the plates out. I then repeated this but done a spread plate and added enough vodka to make agar plates 5% abw. I am about to take a single colony and make a starter in prep for brewing this week and do a side brew with this yeast.



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