D4nny74 wrote:Do you think it needs stepping up again before I use it on a brew ?
How much have you got in there, and what's your batch size and planned OG?
I've just check my post on another forum, from some time ago, and can help a bit on the pitch rate... in theory. On the tour I did, they said they pitch 150Kg of (live) yeast into 28,000L. I would assume that is 'typical', not absolute. So you could call that 123g in 23L for us... but that's very fresh, active yeast. Depending what you'd want to assume may be the cell density you have there, you could make all sorts of guesses as to how much of your culture you need. However, assuming even a moderate density, you'd want well over 200 billion cells to get near their rate. Considering that the best info I have is that they pitch at 15C and hold for 24 hours, before going up 3C or so, I just think that you want the biggest practical starter you can, so step up to a good 2 or 3L. When I used it (before I heard so much about their practices) I pitched too little, too high, and it was estery... although still a strong fermentation. Aerate like heck, too.